Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12971
Title: Promoter hypermethylation of tumor suppressor genes in serum as potential biomarker for the diagnosis of nasopharyngeal carcinoma
Authors: Tian, F
Yip, SP 
Kwong, DLW
Lin, Z
Yang, Z
Wu, VWC 
Keywords: Cell-free circulating DNA
Diagnosis
DNA hypermethylation
MSP
Nasopharyngeal carcinoma
Tumor suppressor genes
Issue Date: 2013
Publisher: Elsevier Sci Ltd
Source: Cancer epidemiology, 2013, v. 37, no. 5, p. 708-713 How to cite?
Journal: Cancer Epidemiology 
Abstract: Purpose: Promoter hypermethylation of tumor suppressor genes may serve as a promising biomarker for the diagnosis of cancer. Cell-free circulating DNA (cf-DNA) shares hypermethylation status with primary tumors. This study investigated promoter hypermethylation of five tumor suppressor genes as markers in the detection of nasopharyngeal carcinoma (NPC) in serum samples. Methods: cf-DNA was extracted from serum collected from 40 NPC patients and 41 age- and sex-matched healthy subjects. The promoter hypermethylation status of the five genes (RASSF1, CDKN2A, DLEC1, DAPK1 and UCHL1) was assessed by methylation-specific PCR after sodium bisulfite conversion. Differences in the methylation status of these five genes between NPC patients and healthy subjects were compared. Results: The concentration of cf-DNA in the serum of NPC patients was significantly higher than that in normal controls. The five tumor suppressor genes - RASSF1, CDKN2A, DLEC1, DAPK1 and UCHL1 - were found to be methylated in 17.5%, 22.5%, 25.0%, 51.4% and 64.9% of patients, respectively. The combination of four-gene marker - CDKN2A, DLEC1, DAPK1 and UCHL1 - had the highest sensitivity and specificity in predicting NPC. Conclusion: Screening DNA hypermethylation of tumor suppressor genes in serum was a promising approach for the diagnosis of NPC.
URI: http://hdl.handle.net/10397/12971
ISSN: 1877-7821
DOI: 10.1016/j.canep.2013.05.012
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