Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12764
Title: Estrogen deficiency-induced Ca balance impairment is associated with decrease in expression of epithelial Ca transport proteins in aged female rats
Authors: Dong, XL
Zhang, Y
Wong, MS 
Keywords: Ca balance
Epithelial Ca transport proteins
Estrogen deficiency
High Ca diet
Low Ca diet
Issue Date: 2014
Publisher: Pergamon-Elsevier Science Ltd
Source: Life sciences, 2014, v. 96, no. 41671, p. 26-32 How to cite?
Journal: Life Sciences 
Abstract: Aims The study is designed to determine whether estrogen and vitamin D endocrine systems interact to regulate calcium (Ca) balance as well as changes in mRNA expression of epithelial Ca transport proteins involved in intestinal and renal Ca transport in aging animals in response to ovariectomy and low dietary Ca intake. Main methods Eleven-month-old female sham or ovariectomized (OVX) rats were divided into four groups and fed with either a low-Ca (LCD; 0.1% Ca, 0.65% P) or a high-Ca (HCD; 1.2% Ca, 0.65% P) diet for 12 weeks. Ca balance and mRNA expression of Ca transport proteins in the intestine and kidney from rats were systematically studied. Key findings OVX rats fed with LCD resulted in a negative Ca balance. LCD suppressed serum Ca in OVX but not sham rats, resulting in an induction of serum PTH and 1,25(OH)2D3 levels. The surge in serum 1,25(OH)2D3 levels in LCD-fed OVX rats was associated with an increase in mRNA expression of intestinal transient receptor potential cation channel (TRPV6) and calbindin D9k (CaBP9k) as well as renal vitamin D receptor (VDR), but such an induction was unable to restore Ca balance in vivo. In contrast, the negative Ca balance was associated with suppression of intestinal plasma membrane Ca pump (PMCA1b) and renal transient receptor potential cation channel (TRPV5), calbindin D28k (CaBP28k) and PMCA1b mRNA expression in aged OVX rats. Significance Negative Ca balance in aged female OVX rats is associated with estrogen-dependent and vitamin D-independent downregulation of epithelial Ca transport protein mRNA expression.
URI: http://hdl.handle.net/10397/12764
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2013.12.025
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