Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12749
Title: Genistein modulates the effects of parathyroid hormone in human osteoblastic SaOS-2 cells
Authors: Chen, WF
Wong, MS 
Keywords: Alkaline phosphatase
Genistein
Osteoprotegrin
Parathyroid hormone
Receptor activator of nuclear factor-κB ligand
Issue Date: 2006
Publisher: Cambridge University Press
Source: British journal of nutrition, 2006, v. 95, no. 6, p. 1039-1047 How to cite?
Journal: British journal of nutrition 
Abstract: Genistein and parathyroid hormone (PTH) are anabolic agents that stimulate bone formation through their direct actions in osteoblastic cells. In the present study, we aimed to determine whether genistein modulates the actions of PTH in human osteoblastic SaOS-2 cells in an oestrogen-depleted condition. The present results showed that genistein (10-8 to 10-6 M) induced alkaline phosphatase (ALP activity and osteoprotegrin (OPG) expression in SaOS-2 cells in a dose-dependent manner. These effects could be completely abolished by co-treatment with oestrogen antagonist ICI 182780 (7α-[9-[(4,4,5,5,5-pentafluoropentyl sulfonyl]nonyl]-estra-1,3,5(10)- triene-3,17β-diol). Genistein (at 1 μM) could stimulate the mRNA expression of receptor activator of NF-κB ligand (RANKL). As OPG and RANKL are known to modulate osteoclastogenesis, the ability of genistein to modulate OPG and RANKL expression in SaOS-2 cells suggested that it might modulate osteoclastogenesis through its direct actions on osteoblastic cells. PTH (at 10 nM) stimulated ALP activity, induced RANKL mRNA expression and suppressed OPG mRNA expression in SaOS-2 cells, confirming its bi-directional effects on osteoblastic cells. Pre-treatment of SaOS-2 cells with genistein and oestrogen not only enhanced PTH-induced ALP activity, but also attenuated PTH up regulation of RANKL mRNA expression and PTH down regulation of OPG mRNA expression. Taken together, the present study provides the first evidence that genistein could modulate the actions of PTH in human osteoblastic SaOS-2 cells in an oestrogen-depleted condition.
URI: http://hdl.handle.net/10397/12749
ISSN: 0007-1145
EISSN: 1475-2662
DOI: 10.1079/BJN20061735
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

21
Last Week
0
Last month
1
Citations as of Aug 20, 2017

WEB OF SCIENCETM
Citations

15
Last Week
0
Last month
1
Citations as of Aug 20, 2017

Page view(s)

31
Last Week
0
Last month
Checked on Aug 20, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.