Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12583
Title: Detection of heavy metal toxicity using cardiac cell-based biosensor
Authors: Liu, Q
Cai, H
Xu, Y
Xiao, L
Yang, M 
Wang, P
Keywords: Cardiomyocyte
Cell-based biosensor
Heavy metal ion
Light-addressable potentiometric sensor
Toxicity
Issue Date: 2007
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2007, v. 22, no. 12, p. 3224-3229 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: Biosensors incorporating mammalian cells have a distinct advantage of responding in a manner which offers insight into the physiological effect of an analyte. To investigate the potential applications of cell-based biosensors on heavy metal toxicity detection, a novel biosensor for monitoring electrophysiological activity was developed by light-addressable potentiometric sensor (LAPS). Extracellular field potentials of spontaneously beating cardiomyocytes could be recorded by LAPS in the range of 20 μV to nearly 40 μV with frequency of 0.5-3 Hz. After exposed to different heavy metal ions (Hg 2+, Pb 2+, Cd 2+, Fe 3+, Cu 2+, Zn 2+; in concentration of 10 μM), cardiomyocytes demonstrated characteristic changes in terms of beating frequency, amplitude and duration under the different toxic effects of ions in less than 15 min. This study suggests that, with the physiological monitoring, it is possible to use the cardiac cell-based biosensor to study acute and eventually chronic toxicities induced by heavy metal ions in a long-term and no-invasive way.
URI: http://hdl.handle.net/10397/12583
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/j.bios.2007.03.005
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