Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12522
Title: Prevention and treatment of allergic inflammation by an Fcγ-Der f2 fusion protein in a murine model of dust mite-induced asthma
Authors: Lin, LH
Zheng, P
Yuen, JWM 
Wang, J
Zhou, J
Kong, CQ
Peng, X
Li, J
Li, L
Keywords: Allergic asthma mouse
Fcγ-Der f2 fusion protein
FcγRIIb
FcεRI
Immunomodulation
Issue Date: 2012
Publisher: Humana Press Inc
Source: Immunologic research, 2012, v. 52, no. 3, p. 276-283 How to cite?
Journal: Immunologic Research 
Abstract: The immunoglobulin E (IgE) high-affinity receptor FcεRI expressed on mast cells and basophils plays a critical role in triggering allergic disease. The co-aggregation of the FcεRI and FcγRIIb receptors is inhibitory to FcεRI signaling and holds great potential for the treatment of IgE-mediated allergies. In China, Dermatophagoides farinae is a common anaphylaxis trigger. Therefore, in this study, the FcγRIIb-mediated immunomodulating activity of recombinant Fcγ-Der f2 fusion protein was tested in a Der f2-allergic murine model. Following the treatment, bronchoalveolar lavage fluid (BALF) was collected to measure the expression of several Th1/Th2-type cytokines (IL-5, TNF-α, IL-12p70, IL-4, IL-10, IFN-γ and IL-18) and histamine, while blood was used to detect the specific IgE and IgG-types anti-Der f2 antibodies, for measurement. In contrast to the saline-treated allergic mice, the levels of Der f2-specific IgE, cytokines and histamine were lowered in the Fcγ-Der f2-treated allergic mice, in addition to the rare inflammatory cell infiltration in the airways and blood vessels revealed by histopathological examination. The recombinant Fcγ-Der f2 protein was demonstrated to function as an effective immunotherapeutic agent, suggesting that chimeric human Fcγ-allergen proteins could be used in the development of antigen-specific immunotherapy for human allergic diseases.
URI: http://hdl.handle.net/10397/12522
DOI: 10.1007/s12026-012-8339-x
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