Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/12052
Title: Improved detection of phosphopeptides by negative ion matrix-assisted laser desorption/ionization mass spectrometry using a proton sponge co-matrix
Authors: Zhang, S
Yao, ZP 
Keywords: MALDI
Mass spectrometry
Matrix
Negative ion
Phosphopeptides
Proton sponge
Issue Date: 2012
Publisher: Elsevier
Source: Analytica chimica acta, 2012, v. 711, p. 77-82 How to cite?
Journal: Analytica chimica acta 
Abstract: Analysis of phosphopeptides is an important task in proteomic studies. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a technique very commonly used for such a purpose. Analysis of phosphopeptides by MALDI-MS is, however, still a challenging task due to the low ionization efficiency of phosphopeptides. In this study, we reported that by using a proton sponge 1,8-bis(dimethyl-amino)naphthalene (DMAN) as a co-matrix, detection of phosphopeptides by negative ion MALDI-MS could be greatly improved. Combination of DMAN with another matrix 6-aza-2-thiothymine (ATT) and additive diammonium hydrogen citrate (DHC) allowed much lower limit of detection, significantly reduced signal suppression effects and improved position-to-position reproducibility for detection of phosphopeptides by negative ion MALDI-MS. Potential applications of the matrix system in qualification of phosphopeptides and analysis of proteolytic digests of phosphorylated proteins were also demonstrated in this study.
URI: http://hdl.handle.net/10397/12052
ISSN: 0003-2670
DOI: 10.1016/j.aca.2011.10.060
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