Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/11657
Title: Identification of a tumor suppressive critical region mapping to 3p14. 2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9
Authors: Lo, PHY
Leung, ACC
Kwok, CYC
Cheung, WSY
Ko, JMY
Yang, LC
Law, S
Wang, LD
Li, J
Stanbridge, EJ
Srivastava, G
Tang, JCO 
Tsao, SW
Lung, ML
Keywords: Esophageal carcinoma
Tumor suppressor gene
Chromosome 3
Microcell-mediated chromosome transfer
ADAMTS9
Issue Date: 2007
Source: Oncogene, 2007, v. 26, no. 1, p. 148-157 How to cite?
Journal: Oncogene 
Abstract: A gene critical to esophageal cancer has been identified. Functional studies using microcell-mediated chromosome transfer of intact and truncated donor chromosomes 3 into an esophageal cancer cell line and nude mouse tumorigenicity assays were used to identify a 1.61 Mb tumor suppressive critical region (CR) mapping to chromosome 3p14.2. This CR is bounded by D3S1600 and D3S1285 microsatellite markers. One candidate tumor suppressor gene, ADAMTS9, maps to this CR. Further studies showed normal expression levels of this gene in tumorsuppressed microcell hybrids, levels that were much higher than observed in the recipient cells. Complete loss or downregulation of ADAMTS9 gene expression was found in 15 out of 16 esophageal carcinoma cell lines. Promoter hypermethylation was detected in the cell lines that do not express this gene. Re-expression of ADAMTS9 was observed after demethylation drug treatment, confirming that hypermethylation is involved in gene downregulation. Downregulation of ADAMTS9 was also found in 43.5 and 47.6% of primary esophageal tumor tissues from Hong Kong and from the high-risk region of Henan, respectively. Thus, this study identifies and provides functional evidence for a CR associated withtumor suppression on 3p14.2 and provides the first evidence that ADAMTS9, mapping to this region, may contribute to esophageal cancer development.
URI: http://hdl.handle.net/10397/11657
ISSN: 0950-9232
DOI: 10.1038/sj.onc.1209767
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

53
Last Week
0
Last month
0
Citations as of May 27, 2017

WEB OF SCIENCETM
Citations

51
Last Week
0
Last month
0
Citations as of May 21, 2017

Page view(s)

33
Last Week
3
Last month
Checked on May 28, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.