Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/11634
Title: Engineering Clostridia Neurotoxins with elevated catalytic activity
Authors: Guo, J
Pan, X
Zhao, Y 
Chen, S 
Keywords: Botulinum Neurotoxin B
Elevated activity
Engineering
Light chain
TeNT
VAMP2
Issue Date: 2013
Publisher: Pergamon-Elsevier Science Ltd
Source: Toxicon, 2013, v. 74, p. 158-166 How to cite?
Journal: Toxicon 
Abstract: BoNT/B and TeNT cleave substrate VAMP2 at the same scissile bond, yet these two toxins showed different efficiency on substrate hydrolysis and had different requirements for the recognition of P2' site of VAMP2, E78. These differences may be due to their different composition of their substrate recognition pockets in the active site. Swapping of LC/T S1' pocket residue, L230, with the corresponding isoleucine in LC/B increased LC/T activity by ~25 fold, while swapping of LC/B S1' pocket residue, S201, with the corresponding proline in LC/T increased LC/B activity by ~10 fold. Optimization of both S1 and S1' pocket residues of LC/T, LC/T (K168E, L230I) elevated LC/T activity by more than 100-fold. The highly active LC/T derivative engineered in this study has the potential to be used as a more effective tool to study mechanisms of exocytosis in central neuron. The LC/B derivative with elevated activity has the potential to be developed into novel therapy to minimize the impact of immunoresistance during BoNT/B therapy.
URI: http://hdl.handle.net/10397/11634
ISSN: 0041-0101
DOI: 10.1016/j.toxicon.2013.08.055
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