Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/11616
Title: Human cytomegalovirus induces caspase-dependent apoptosis of megakaryocytic CHRF-288-11 cells by activating the JNK pathway
Authors: Dou, J
Li, X
Cai, Y
Chen, H
Zhu, S
Wang, Q
Zou, X
Mei, Y
Yang, Q
Li, W
Han, Y 
Keywords: Apoptosis
C-Jun terminal kinase
Caspase
Human cytomegalovirus
Thrombocytopenia
Issue Date: 2010
Publisher: Springer Tokyo
Source: International journal of hematology, 2010, v. 91, no. 4, p. 620-629 How to cite?
Journal: International Journal of Hematology 
Abstract: Human cytomegalovirus (HCMV) infection is usually implicated in thrombocytopenia occurring in newborns and immunocompromised patients. However, the underlying mechanisms remain elusive. This study was conducted to investigate the effects of HCMV infection on the viability of megakaryocytic CHRF-288-11 cells and the underlying mechanisms involved. RT-PCR for determining mRNA expression of HCMV immediate early gene 1 and Western blot for measuring protein expression of late HCMV gene pp65 showed that CHRF-288-11 cells were susceptible to HCMV infection. HCMV infection reduced the viability of CHRF-288-11 cells via apoptosis in a doseand time-dependent manner. Both caspase 3 and c-Jun terminal kinase (JNK) signaling pathway were activated in the HCMV-treated CHRF-288-11 cells. z-DEVD-fmk (a caspase inhibitor) and SP600125 (a JNK inhibitor) significantly prevented the death of CHRF-288-11 cells induced by HCMV, respectively. Furthermore, inhibition of JNK activity could reduce the formation of active caspase 3 induced by HCMV. Interestingly, the co-application of antivirus drug ganciclovir and SP600125 synergistically prevented the death of CHRF-288-11 cells induced by HCMV. Collectively, these findings suggest that HCMV infection may induce the caspase-dependent apoptosis of megakaryocytic CHRF-288-11 cells by the activation of JNK signaling pathway.
URI: http://hdl.handle.net/10397/11616
DOI: 10.1007/s12185-010-0560-6
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