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Title: Importance of early detection of vancomycin-resistant subpopulations in apparently susceptible meticillin-resistant Staphylococcus aureus clinical isolates
Authors: O'Donoghue, MM 
Doddangoudar, VC
Tsang, DNC
Boost, MV
Keywords: HVISA
Spiral gradient endpoint technique
Vancomycin non-susceptibility
Issue Date: 2015
Publisher: Elsevier Ltd
Source: Journal of global antimicrobial resistance, 2015, v. 3, no. 2, p. 64-68 How to cite?
Journal: Journal of Global Antimicrobial Resistance 
Abstract: This study aimed to investigate the presence of vancomycin-non-susceptible subpopulations in apparently susceptible meticillin-resistant Staphylococcus aureus (MRSA) and the ability of these isolates to develop into homogeneously resistant strains. Vancomycin MICs of 200 clinical MRSA isolates were determined using agar dilution (AD) and spiral gradient endpoint (SGE) technique. Isolates with an MIC. ≤. 2. mg/L but displaying subpopulations with an MIC. >. 2. mg/L by SGE were re-tested by Etest and PAP-AUC and were incubated with 2. mg/L vancomycin for 2 weeks. MIC testing was repeated weekly by AD, Etest and SGE to observe progression to non-susceptibility. A total of 17.5% and 16.0% of isolates were non-susceptible to vancomycin (MIC. >. 2. mg/L) by SGE and AD, respectively. Eight isolates (4%) displayed a resistant subpopulation; five met the definition of hVISA by PAP-AUC. The initial Etest MIC for these isolates was 2. mg/L, but resistant subpopulations were observed in only three isolates on prolonged incubation. MICs of all eight isolates increased rapidly in the presence of vancomycin, reaching ≥3.0. mg/L by Day 7 and ≥4. mg/L after 14 days by all three methods. The prevalence of vancomycin-non-susceptible MRSA was high, and non-susceptibility developed rapidly in seemingly susceptible isolates with covert subpopulations. These were effectively detected by SGE. With increasing reports of vancomycin clinical failure, early detection of potentially non-susceptible isolates before or early in vancomycin therapy is essential to avoid further resistance development and poor clinical outcomes. SGE offers a novel and cost-effective technique for detection of potentially non-susceptible strains.
ISSN: 1444-0938
DOI: 10.1016/j.jgar.2015.01.007
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