Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/11191
Title: Enhancement of tanshinone production in Salvia miltiorrhiza hairy root culture by Ag+ elicitation and nutrient feeding
Authors: Zhang, CH
Yan, Q
Cheuk, WK
Wu, JY 
Keywords: Hairy root culture
Salvia miltiorrhiza
Lamiaceae
Tanshinones
Ag+ elicitation
Nutrient feeding
Issue Date: 2004
Publisher: Georg Thieme Verlag Kg
Source: Planta medica, 2004, v. 70, no. 2, p. 147-151 How to cite?
Journal: Planta Medica 
Abstract: Ag+ was employed as an abiotic elicitor to stimulate the secondary metabolite production in hairy root Culture of Salvia miltiorrhiza, a valuable herbal plant. The addition of Ag2S2O3 at 15 - 40 muM to the culture between 12 and 22 days post inoculation resulted in more than 2-fold increase in the yields of the three diterpenoid tanshinones, tanshinone I, tanshinone IIA and cryptotanshinone. This stimulating effect was dependent on the Ag+ dose, the day of Ag+ addition to the culture and the tanshinone species, while the total yield of the three tanshinones (TT) was mainly dependent on the Ag+ dose, with the highest being attained at 30 muM. The Ag+ treatment also caused a close-dependent inhibition of hairy root growth. Sucrose feeding or medium renewal before the addition of Ag+ to the culture effectively prevented the growth inhibition, and significantly increased the biomass concentration and volumetric tanshinone yield. With combined medium renewal and Ag+ treatment, in particular, the TT yield was increased 6.6-fold relative to that of the control (55.7 mg/L versus 7.3 mg/L). The elicitor function of Ag+ in the hairy root culture was validated by its ability to induce the characteristic elicitor responses of plants, the increase in cross-cell membrane ion fluxes and the production of reactive oxygen species.
URI: http://hdl.handle.net/10397/11191
ISSN: 0032-0943
DOI: 10.1055/s-2004-815492
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

70
Last Week
4
Last month
Citations as of Sep 18, 2017

WEB OF SCIENCETM
Citations

49
Last Week
0
Last month
1
Citations as of Sep 14, 2017

Page view(s)

49
Last Week
1
Last month
Checked on Sep 18, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.