Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10768
Title: Genistein enhances insulin-like growth factor signaling pathway in human breast cancer (MCF-7) cells
Authors: Chen, WF
Wong, MS 
Issue Date: 2004
Publisher: Endocrine Soc
Source: Journal of clinical endocrinology and metabolism, 2004, v. 89, no. 5, p. 2351-2359 How to cite?
Journal: Journal of Clinical Endocrinology and Metabolism 
Abstract: Physiological concentration of genistein, a natural isoflavonoid phytoestrogen, stimulates human breast cancer (MCF-7) cells proliferation. In this study, we hypothesize that low concentration of genistein mimics the action of 17β-estradiol in stimulation of MCF-7 cell growth by enhancement of IGF-I signaling pathway. Genistein, at 1 μM, stimulated the growth of MCF-7 cells. Cell cycle analysis showed that 1 μM genistein significantly increased the S phase and decreased the GOG1 phase of MCF-7 cells. The protein and mRNA expression of IGF-I receptor (IGF-IR) and insulin receptor substrate (IRS)-1, but not Src homology/collagen protein, increased in response to 1 μM genistein in a time-dependent manner. These effects could be completely abolished by cotreatment of MCF-7 cells with estrogen antagonist ICI 182,780 (1 μM) and tamoxifen (0.1 μM). Our results also showed that genistein induction of IGF-IR and IRS-1 expression resulted in enhanced tyrosine phosphorylation of IGF-IR and IRS-1 on IGF-I stimulation. Taken together, these data provide the first evidence that the IGF-IR pathway is involved in the proliferative effect of low-dose genistein in MCF-7 cells.
URI: http://hdl.handle.net/10397/10768
ISSN: 0021-972X
DOI: 10.1210/jc.2003-032065
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

62
Last Week
0
Last month
1
Citations as of Jun 16, 2017

WEB OF SCIENCETM
Citations

55
Last Week
0
Last month
Citations as of Jun 22, 2017

Page view(s)

25
Last Week
0
Last month
Checked on Jun 18, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.