Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10739
Title: Maternal low-level lead exposure reduces the expression of PSA-NCAM and the activity of sialyltransferase in the hippocampi of neonatal rat pups
Authors: Hu, Q
Fu, H
Ren, T
Wang, S
Zhou, W
Song, H
Han, Y 
Dong, S
Keywords: Hippocampus
Lead
Neonatal rat pups
Polysialyltransferase
PSA-NCAM
Issue Date: 2008
Publisher: Elsevier Science Bv
Source: Neurotoxicology, 2008, v. 29, no. 4, p. 675-681 How to cite?
Journal: NeuroToxicology 
Abstract: Highly polysialylated neural cell adhesion molecule (PSA-NCAM) is transiently expressed specifically in newly generated cells, and is important for cell migration and neurite outgrowth. Developmental lead (Pb) exposure has been considered to affect the expression of PSA-NCAM, which contributes to the neurotoxicity of Pb exposure. However, the effect of maternal low-level Pb exposure on the expression of PSA-NCAM in neonatal rat pups has not been reported. In the present study, female Wistar rats were exposed to vehicle or different dosages of lead chloride (0.5-4 mM PbCl 2) 2 weeks before and during pregnancy. This exposure protocol resulted in neonatal rat pups blood Pb levels up to 12.12 ± 0.38 μg/dl, and hippocampal Pb levels up to 9.22 ± 0.81 μg/g at postnatal day 1 (PND 1). Immunohistochemistry analysis and Western blot analysis revealed that the expressions of PSA-NCAM and NCAM in the hippocampi of neonatal rat pups at PND 1 were significantly reduced by the maternal low-level Pb exposures. Furthermore, the mRNA levels of NCAM and polysialyltransferases (STX and PST), measured by the fluorescent real-time quantitative RT-PCR, dosage-dependently and significantly decreased by 13.26-37.62%, 25.17-59.67%, and 10.78-47.81%, respectively. In addition, the sialyltransferase activity in neonatal rat pups was significantly reduced by 6.23-32.50% in the presence of the low-level Pb exposure, too. Taken together, these results suggest that maternal low-level Pb exposure reduces the expression of PSA-NCAM, NCAM, and the activity of sialyltransferase in the hippocampi of neonatal rat pups, which might contribute to the learning and memory impairments in the developmental pups following maternal low-level Pb exposure.
URI: http://hdl.handle.net/10397/10739
DOI: 10.1016/j.neuro.2008.04.002
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

27
Last Week
0
Last month
2
Citations as of Aug 20, 2017

WEB OF SCIENCETM
Citations

26
Last Week
0
Last month
1
Citations as of Aug 16, 2017

Page view(s)

52
Last Week
0
Last month
Checked on Aug 20, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.