Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10610
Title: Label-free enumeration of colorectal cancer cells from lymphocytes performed at a high cell-loading density by using interdigitated ring-array microelectrodes
Authors: Xing, X
Poon, RYC
Wong, CSC 
Yobas, L
Keywords: Cancer
Cells
Dielectrophoresis
Electrode
Microfluidics
Silicon
Issue Date: 2014
Publisher: Elsevier
Source: Biosensors and bioelectronics, 2014, v. 61, p. 434-442 How to cite?
Journal: Biosensors and bioelectronics 
Abstract: We report the label-free enumeration of human colorectal-carcinoma cells from blood lymphocytes by using interdigitated ring-array microelectrodes; this enumeration was based on the dielectrophoretic selection of cells. Because of the novel design of the device, a continuous flow of cells is uniformly distributed into parallel streams through 300 rings (~40μm in diameter each) that are integrated into the electrode digits. Using this array, 82% of cancer cells were recovered and 99% of blood lymphocytes were removed. Most of the cancer cells recovered were viable (94%) and could be cultivated for >8 days, during which period they retained their normal cell morphology and proliferation rates. The recovery rate correlated closely with cancer-cell loadings in spiked samples and this relationship was linear over a range of at least 2 orders of magnitude. Importantly, because of the 3D structure of the rings, these results were obtained at a high cell-loading concentration (107cells/mL). The rings could be further optimized for use in accurate label-free identification and measurement of circulating tumor cells in cancer research and disease management.
URI: http://hdl.handle.net/10397/10610
ISSN: 0956-5663
EISSN: 1873-4235
DOI: 10.1016/j.bios.2014.05.054
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