Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10376
Title: Hypothermia attenuates protective effects of ginkgolides on astrocytes from ischemia/reperfusion injury
Authors: Fang, D
Ming, QZ
Li, Z
Mei, WX
Ya, K
Keywords: Astrocytes
Ginkgolides
Hypothermia
Hypoxia-inducible factor-1alpha (HIF-1alpha)
Ischemia and reperfusion injury
Protective role
Issue Date: 2009
Publisher: Pergamon-Elsevier Science Ltd
Source: Neurochemistry international, 2009, v. 55, no. 4, p. 181-186 How to cite?
Journal: Neurochemistry International 
Abstract: The neuroprotective roles of both hypothermia and ginkgolides have been well confirmed. We first examined whether hypothermia (32 or 28 °C) or ginkgolides have a protective effect on astrocytes against ischemia and reperfusion-induced injury. We demonstrated that ginkgolides, but not hypothermia, have a significantly time- and concentration-dependent protective role in ischemic astrocytes. We then investigated whether co-treatment with hypothermia and ginkgolides has a synergistic role to protect astrocytes against ischemia and reperfusion-induced injury. Cells were incubated with 18.75, 37.5 or 75 μg/ml of ginkgolides at 37, 32 or 28 °C for 24, 48 or 72 h before exposure to ischemia (24 h) and then reperfusion (24 h). Data showed that the co-treatment induced a significant decrease, rather than an increase as we had expected, in their cellular viabilities and anti-apoptotic abilities as compared with the cells treated by ginkgolides only. Western blot analysis demonstrated that hypothermia (32 or 28 °C for 24 h) has no effect on the expression of Hypoxia-inducible factor-1alpha (HIF-1alpha) protein, suggesting that HIF-1alpha is not associated with the adverse effect of hypothermia on ginkgolides. The findings imply the importance of further investigating the effects of hypothermia on the pharmacological role or therapeutic efficacy of drugs commonly used clinically.
URI: http://hdl.handle.net/10397/10376
DOI: 10.1016/j.neuint.2008.12.009
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