Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10328
Title: Upconversion nanoparticles conjugated with Gd3+-DOTA and RGD for targeted dual-modality imaging of brain tumor xenografts
Authors: Jin, J
Xu, Z
Zhang, Y
Gu, YJ
Lam, MHW
Wong, WT 
Keywords: Dual-modality
Fluorescence-guided surgery
Glioblastoma
Targeted imaging
Upconversion
Issue Date: 2013
Publisher: Wiley-VCH
Source: Advanced healthcare materials, 2013, v. 2, no. 11, p. 1501-1512 How to cite?
Journal: Advanced healthcare materials 
Abstract: Glioblastoma multiforme (GBM) is the most common and malignant form of primary brain tumors in human. Small molecular magnetic resonance imaging (MRI) contrast agents are used for GBM diagnosis. However, conventional contrast agents have several limitations, such as low T1 relaxivity, short circulation half lives and absence of tumor targeting. Herein, we develop an upconversion nanoprobe labeled with Gd3+-DOTA and RGD (UCNP-Gd-RGD) for dual-modality imaging of glioblastoma. The preparation of UCNP-Gd-RGD starts with amine-functional upconversion nanoparticle core, followed by PEGylation, Gd3+DOTA conjugation and RGD labeling. The obtained UCNP-Gd-RGD has improved colloidal stability and reduced cytotoxicity compared with the UCNP core counterpart. Meanwhile, UCNP-Gd-RGD shows strong upconversion luminescence in deep-red region and three times enhancement of T1 relaxivity over Gd3+DOTA. Due to the recognition between UCNP-Gd-RGD and integrin αvβ3 receptors, the nanoprobe specifically binds to U87MG cells, as evidenced by confocal microscopy and quantified by ICP-MS. Furthermore, UCNP-Gd-RGD demonstrates a preferential retention in subcutaneous U87MG tumor xenograft as shown in both in vivo upconversion fluorescence/MR imaging studies and ex vivo analysis. UCNP-Gd-RGD, conjugated with numerous RGD peptide and T1 contrast enhancing molecules, is promising for MR imaging of glioblastoma and delineating the tumor boundary before surgery. In addition, NIR-to-red upconversion characteristic of UCNP-Gd-RGD facilitates its potential intra-operative use for fluorescence-guided tumor resection.
URI: http://hdl.handle.net/10397/10328
ISSN: 2192-2640
EISSN: 2192-2659
DOI: 10.1002/adhm.201300102
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

27
Last Week
0
Last month
1
Citations as of Aug 20, 2017

WEB OF SCIENCETM
Citations

26
Last Week
0
Last month
1
Citations as of Aug 21, 2017

Page view(s)

40
Last Week
1
Last month
Checked on Aug 20, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.