Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/10268
Title: An optical biosensor for the rapid determination of glucose in human serum
Authors: Wang, XD
Zhou, TY
Chen, X
Wong, KY 
Wang, XR
Issue Date: 2008
Source: Sensors and actuators. B, Chemical, 2008, v. 129, no. 2, p. 866-873
Abstract: An optical glucose biosensor based on the immobilization of glucose oxidase in a sensing film was developed. The sensing film consisted of an organically modified silicate film embedded with tri(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) perchlorate and a polyvinyl alcohol sol-gel matrix with immobilized glucose oxidase. A kinetic curve simulation method was employed for glucose measurements, as a result of which the measurement time was less than 1 min for each sample analysis. The detection limit (S/N = 3) of the glucose optical sensor was 3.6 × 10-6 mol L-1 with linear ranges from 0.00 to 0.50 mmol L-1 (Y = 358.25X-2.93, R2 = 0.9954) and 0.50 to 3.00 mmol L-1 (Y = 430.12X-46.43, R2 = 0.9972). In addition, the appearance of the sensing film, and effects of the amounts of immobilized enzyme, pH, temperature, ionic strength, and co-existing substances (including heavy metal ions and organic compounds) were investigated. The glucose values estimated by this optical biosensor correlated well with those determined using the conventional method for human serum samples.
Keywords: Glucose optical biosensor
Glucose oxidase
Kinetic measurement
ORMOSIL-PVA
Serum
Publisher: Elsevier
Journal: Sensors and actuators. B, Chemical 
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2007.09.079
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